| 128 | J. Mitsuko Mahoney (CENW) def. Mary Breitbach (LORWTEN) 2-6, 7-6 (10-4), 11-9 |
| 128 | Brooke Haddorff (GACW) def. Monica Zink (SCU) 6-0, 6-3 |
| 128 | Katherine Trifilio (CU) def. Addison Leasure (COEW) 6-1, 6-4 |
| 128 | Evelyn Taylor (EC) def. Natalie Kaiser (WARWT) 6-0, 6-4 |
| 128 | Montserrat Ramirez (LUTW) def. Sarah Ferguson (UWW) 4-5, 6-7 (5-0) |
| 128 | Faith Simon (SCU) def. Elizabeth Trevathan (MAC) 6-1, 6-3 |
| 128 | Piper Brown (CAR) def. Courtney Nuest (BU) 6-3, 6-4 |
| 128 | Jane Spading (STO) def. Andreina Tejada (UWSW) 7-6 (0-3), 6-0 |
| 128 | Quinlyn Mack (UWSPW) def. Caitlyn Stadter (SCU) 6-4, 7-6 (0-1) |
| 128 | R. Queiroga Tavares (LUTW) def. Alli Hagness (COEW) 6-1, 7-6 (0-3) |
| 128 | Liv Herzog (UWEC) def. Sydney Marin (GCW) 6-7 (2-0), 5-7 |
| 128 | Lauren Lindow (UWL) def. Clara Mast (CAR) 6-1, 6-2 |
| 128 | Alexandra Berns (STO) def. Alexis Budzinski (UWRF) 6-3, 6-1 |
| 128 | Mac Graves (CCW) def. Greta Nesbit (CSB) 6-3, 6-3 |
| 128 | Claire Keller (BU) def. Hannah Cady (UWL) 6-1, 6-4 |
| 128 | Pavla Yakimova (GACW) def. Riley O'Donnell (LUTW) 6-3, 6-2 |
| 128 | Ava Andrae (UWW) def. Malia McKinnon (BU) 6-4, 6-4 |
| 128 | Tan Mukherjee (GCW) def. Reese Burton (UWEC) 6-3, 6-1 |
| 128 | Maya Brakhage (GACW) def. Katia Bartels (CSS) 6-3, 6-4 |
| 128 | Fiona O'Flaherty (STO) def. Alex Repplier (UWW) 1-6, 6-3, 10-5 |
| 128 | Sydney Wiener (UWRF) def. Kalea Martin (GCW) 6-1, 6-0 |
| 128 | Natasa Pupovac (CU) def. Lisey Langhus (BU) 6-2, 6-0 |
| 128 | Lena Lichau (LUTW) def. Emma Farder (CC) 6-0, 6-0 |
| 128 | Sasha Manojlovic (CART) def. Peyton Morgan (CUW) 6-0, 6-4 |
| 128 | Julia Baber (BU) def. Tristin Jantz (UWSPW) 6-1, 5-7, 10-8 |
| 128 | Julianna Mascardo (LUTW) def. Sophia Jonas (CSB) 6-1, 6-0 |
| 128 | Maia Samuelson (UWL) def. Beatriz Felchak (UWEC) 6-2, 6-2 |
| 128 | Anika Sheth (GCW) def. Emma Thole (BU) 6-4, 7-5 |
| 128 | Alice Tysoe (COEW) def. Dorothy Deans (LORWTEN) 6-1, 2-6, 10-6 |
| 128 | Tansy Graves (CCW) def. Sophia Cosgrove (CSS) 6-4, 1-6, 10-7 |
| 128 | Savannah Haugen (MAC) def. Madi Whalen (CENW) 6-1, 6-0 |
| 128 | Abby Ostermann (LUTW) def. Greta Peterson (CART) 6-1, 6-2 |
| 128 | Ella De Young (GACW) def. Emma Heinert (UWL) 6-0, 6-0 |
| 128 | Crystina Lee (UWW) def. Morgan Dekan (UWEC) 6-2, 6-0 |
| 64 | Cassie Lee (UWW) def. J. Mitsuko Mahoney (CENW) 6-0, 6-0 |
| 64 | Anna Lee (UWEC) def. Brooke Haddorff (GACW) 7-5, 1-6, 10-6 |
| 64 | Shelby Roth (UWL) def. Katherine Trifilio (CU) 6-1, 6-1 |
| 64 | Evelyn Taylor (EC) def. Michaella Sullivan (BU) 6-4, 2-6, 10-7 |
| 64 | Kaya De Bruijn (GACW) def. Sarah Ferguson (UWW) 6-2, 6-1 |
| 64 | Lily Perrin (GCW) def. Giovanna Mendes (CART) 7-5, 6-2 |
| 64 | Julia Maier (CSS) def. Faith Simon (SCU) 6-2, 6-4 |
| 64 | Piper Brown (CAR) def. Jane Spading (STO) 6-1, 6-2 |
| 64 | Xingchen Liu (CART) def. Quinlyn Mack (UWSPW) 6-2, 6-2 |
| 64 | Abby Weaver (UWW) def. R. Queiroga Tavares (LUTW) 7-5, 6-2 |
| 64 | Molly Austin (GACW) def. Sydney Marin (GCW) 6-2, 6-2 |
| 64 | Lauren Lindow (UWL) def. Alexandra Berns (STO) 6-1, 6-1 |
| 64 | Paige Sargent (GCW) def. Mac Graves (CCW) 6-1, 6-1 |
| 64 | Claire Keller (BU) def. Pavla Yakimova (GACW) 6-2, 6-3 |
| 64 | Kiley Pickens (CAR) def. Ava Andrae (UWW) 6-1, 6-4 |
| 64 | Tan Mukherjee (GCW) def. Allison Szalay (GACW) 4-6, 6-3, 10-8 |
| 64 | Sophia Fain (COEW) def. Maya Brakhage (GACW) 1-6, 6-2, 10-7 |
| 64 | Pari Chavan (GCW) def. Fiona O'Flaherty (STO) 6-3, 6-1 |
| 64 | Zoe Rose (CAR) def. Kalea Martin (GCW) 6-4, 6-4 |
| 64 | Samantha Fuchs (UWEC) def. Natasa Pupovac (CU) 7-5, 6-1 |
| 64 | Gracie Ha (UWW) def. Lena Lichau (LUTW) 6-1, 6-1 |
| 64 | Tasha Bailey (UWL) def. Sasha Manojlovic (CART) 6-2, 6-1 |
| 64 | Utsah Kalra (GCW) def. Julia Baber (BU) 6-2, 7-5 |
| 64 | Karina Elvestrom (GACW) def. Julianna Mascardo (LUTW) 6-3, 6-2 |
| 64 | Molly Asfeld (UWW) def. Maia Samuelson (UWL) 7-6 (0-3), 6-4 |
| 64 | Miriam Shteyman (CAR) def. Anika Sheth (GCW) 6-4, 7-5 |
| 64 | Alli Laux (GACW) def. Alice Tysoe (COEW) 6-0, 6-2 |
| 64 | Kelsey Dorr (SCU) def. Tansy Graves (CCW) 6-0, 6-2 |
| 64 | Noor Omar (GACW) def. Savannah Haugen (MAC) 6-3, 6-0 |
| 64 | Olha Zubarieva (STO) def. Abby Ostermann (LUTW) 6-3, 6-1 |
| 64 | Erin Jarvis (GCW) def. Ella De Young (GACW) 3-6, 6-3, 10-4 |
| 64 | Lauryn Douglas (BU) def. Crystina Lee (UWW) 6-0, 6-1 |
| 32 | Cassie Lee (UWW) def. Anna Lee (UWEC) 6-4, 6-0 |
| 32 | Shelby Roth (UWL) def. Evelyn Taylor (EC) 6-4, 6-7 (3-0), 10-8 |
| 32 | Kaya De Bruijn (GACW) def. Lily Perrin (GCW) 6-1, 6-2 |
| 32 | Piper Brown (CAR) def. Julia Maier (CSS) 6-0, 4-6, 12-10 |
| 32 | Xingchen Liu (CART) def. Abby Weaver (UWW) 7-5, 6-2 |
| 32 | Molly Austin (GACW) def. Lauren Lindow (UWL) 6-2, 6-2 |
| 32 | Claire Keller (BU) def. Paige Sargent (GCW) 6-4, 6-4 |
| 32 | Kiley Pickens (CAR) def. Tan Mukherjee (GCW) 6-4, 6-3 |
| 32 | Pari Chavan (GCW) def. Sophia Fain (COEW) 6-0, 7-5 |
| 32 | Samantha Fuchs (UWEC) def. Zoe Rose (CAR) 6-3, 6-3 |
| 32 | Gracie Ha (UWW) def. Tasha Bailey (UWL) 1-6, 7-5, 12-10 |
| 32 | Karina Elvestrom (GACW) def. Utsah Kalra (GCW) 6-2, 7-6 (0-1) |
| 32 | Molly Asfeld (UWW) def. Miriam Shteyman (CAR) 3-6, 6-3, 10-3 |
| 32 | Kelsey Dorr (SCU) def. Alli Laux (GACW) 7-5, 6-1 |
| 32 | Noor Omar (GACW) def. Olha Zubarieva (STO) 6-3, 6-4 |
| 32 | Lauryn Douglas (BU) def. Erin Jarvis (GCW) 6-4, 6-1 |
| 16 | Cassie Lee (UWW) def. Shelby Roth (UWL) 6-1, 6-3 |
| 16 | Piper Brown (CAR) def. Kaya De Bruijn (GACW) 5-7, 6-2, 12-10 |
| 16 | Xingchen Liu (CART) def. Molly Austin (GACW) 7-5, 6-2 |
| 16 | Claire Keller (BU) def. Kiley Pickens (CAR) 6-3, 6-3 |
| 16 | Pari Chavan (GCW) def. Samantha Fuchs (UWEC) 3-6, 6-3, 10-3 |
| 16 | Karina Elvestrom (GACW) def. Gracie Ha (UWW) 6-3, 6-0 |
| 16 | Kelsey Dorr (SCU) def. Molly Asfeld (UWW) 6-2, 6-0 |
| 16 | Lauryn Douglas (BU) def. Noor Omar (GACW) 6-1, 6-0 |
| QF | Cassie Lee (UWW) def. Piper Brown (CAR) 5-7, 6-1, 6-0 |
| QF | Xingchen Liu (CART) def. Claire Keller (BU) 6-2, 6-2 |
| QF | Karina Elvestrom (GACW) def. Pari Chavan (GCW) 4-6, 6-4, 6-3 |
| QF | Kelsey Dorr (SCU) def. Lauryn Douglas (BU) 7-5, 6-2 |
| SF | Xingchen Liu (CART) def. Cassie Lee (UWW) 6-2, 6-4 |
| SF | Karina Elvestrom (GACW) def. Kelsey Dorr (SCU) 6-1, 6-3 |
| F | Karina Elvestrom (GACW) def. Xingchen Liu (CART) 1-6, 6-4, 6-4 |
