| 128 | R. Queiroga Taveres (LUTW) def. Natasa Pupovac (CU) 5-7, 6-0, 10-5 |
| Meg Ulrich (MAC) def. Natalia Smith (LUTW) 6-2, 6-2 |
| Emma Thole (BU) def. Abby Struble (LUTW) 6-1, 6-3 |
| Riley O'Donnell (LUTW) def. Ella DeYoung (GAC) 6-2, 4-6, 10-3 |
| Alliandra Hagness (COEW) def. Sophie Geske (UWEC) 6-2, 6-0 |
| Emma Heinert (UWL) def. Becca Vala (CENW) 6-2, 6-0 |
| Mary Breitbach (LORW) def. Tristan Jantz (UWSP) 6-4, 7-6 |
| Tansy Graves (CC) def. Georgia Nissen (WARWT) 7-5, 6-1 |
| Ashley Adkisson (UWW) def. Jennifer Larson (CENW) 6-1, 6-0 |
| Kate Townsend (UWL) def. Bella Tranquilino (SIMPW) 6-3, 6-2 |
| Reese Williams (WARWT) def. Addie Pessian (GRIN) 6-4, 6-3 |
| Brooke Haddorf (GAC) def. Jenna Hernandez (CENW) 6-1, 6-1 |
| Mallory Moen (CONW) def. Natalie Kaiser (WARWT) 6-4, 6-1 |
| Mishell Benitez (SS) def. Madi Whalen (CENW) 6-0, 6-2 |
| Dorothy Deans (LORW) def. Avery Nicholson (UWL) 6-2, 6-7, 10-7 |
| 64 | Gracie Ha (UWW) def. R. Queiroga Taveres (LUTW) 6-1, 6-2 |
| Abby Ostermann (LUTW) def. Hannah Cady (UWL) 7-5, 3-6, 10-6 |
| Michaella Sullivan (BU) def. Julianna Mascardo (LUTW) 1-6, 7-5, 10-8 |
| Sofia Sackett (LUTW) def. Mallory Moen (CONW) 6-2, 3-6, 12-10 |
| Molly Asfeld (UWW) def. Riley O'Donnell (LUTW) 6-4, 6-3 |
| Kelsey Dorr (CSC) def. Alliandra Hagness (COEW) 6-2, 6-1 |
| Anna Lee (UWEC) def. Mary Breitbach (LORW) 6-3, 2-6, 10-6 |
| Sam Fuchs (UWEC) def. Reese Williams (WARWT) 6-0, 6-0 |
| Maddie Schultz (COEW) def. Nichole Henning (GRIN) 6-2, 6-1 |
| Ashley Tarrolly (CSB) def. Dorothy Deans (LORW) 6-1, 6-1 |
| 32 | Ricki Budewitz (CONW) def. Abby Ostermann (LUTW) 6-4, 6-4 |
| Sofia Sackett (LUTW) def. Sydney Presler (UWEC) 6-1, 6-1 |
| Lauryn Douglas (BU) def. Maddie Schultz (COEW) 1-6, 7-6, 10-8 |
| 16 | Molly Austin (GAC) def. Sofia Sackett (LUTW) 6-4, 6-0 |
