| 128 | Kellie Hierl (UWLC) def. Montse Ramirez (LC) 6-3, 4-6, 11-9 |
| 128 | Kellie Ha (UWW) def. Emily Norman (GACW) 6-2, 6-2 |
| 128 | Allie Prokosh (STO) def. Natalie Peterson (SMU) 6-1, 6-1 |
| 128 | Mary Skorich (CCM) def. Amalin Sorajja (MC) 6-3, 1-6, 12-10 |
| 128 | Sofia Walker (SCU) def. Peyton Kopel (CSB) 6-0, 6-0 |
| 128 | Sasha Manojlovic (CAR) def. Lauren Diiulio (LC) 6-4, 7-5 |
| 128 | Lily Perrin (GC) def. Mackenzie Lindow (UWW) 6-1, 6-4 |
| 64 | Sabring Tang (GC) def. Kellie Hierl (UWLC) 6-1, 6-1 |
| 64 | Kellie Ha (UWW) def. Lauryn Douglas (BU) 6-2, 6-2 |
| 64 | Sam Fuchs (UWEC) def. Jenna Hernandez (CENW) 6-1, 6-0 |
| 64 | Cassie Lee (UWW) def. Mary Skorich (CCM) 6-1, 6-1 |
| 64 | Sofia Walker (SCU) def. Sasha Manojlovic (CAR) 6-1, 6-3 |
| 64 | Kayla De Bruijn (GACW) def. Sydney Pressler (UWEC) 6-4, 6-4 |
| 64 | Lily Perrin (GC) def. Julia Maier (SSC) 6-3, 5-7, 10-6 |
| 32 | Sabring Tang (GC) def. Kellie Ha (UWW) 6-1, 6-2 |
| 32 | Sam Fuchs (UWEC) def. Allie Prokosh (STO) 5-7, 6-1, 10-8 |
| 32 | Cassie Lee (UWW) def. Mary Skorich (CCM) 6-1, 6-1 |
| 32 | Sofia Walker (SCU) def. Sasha Manojlovic (CAR) 6-1, 6-3 |
| 32 | Kayla De Bruijn (GACW) def. Lily Perrin (GC) 6-3, 6-1 |
| 16 | Sabring Tang (GC) def. Sam Fuchs (UWEC) 6-1, 6-2 |
| 16 | Cassie Lee (UWW) def. Kayla De Bruijn (GACW) 6-3, 6-3 |
| 8 | Sabring Tang (GC) def. Cassie Lee (UWW) 6-1, 6-3 |
| 4 | Sabring Tang (GC) def. Autumn Bruno (UWW) 3-6, 6-2, 6-2 |
| 128 | Riley O'Donnell (LC) def. Ani Bulbulian (UWLC) 6-2, 6-4 |
| 128 | Julia Baber (BU) def. Avery Munster (MC) 6-4, 6-1 |
| 128 | Gracie Ha (UWW) def. Utsah Kalra (GC) 7-5, 1-6, 10-2 |
| 128 | Morgan Nelson (UWEC) def. Andreina Tejada (UWS) 6-0, 6-0 |
| 128 | Bethany Marshall (UWW) def. Maria Bedford (CSB) 6-2, 6-2 |
| 128 | Alli Laux (GACW) def. Rylee Schurmann (BU) 6-4, 6-1 |
| 128 | Ashley Samuta (CWI) def. Jania Carter-Johnson (COE) 6-3, 6-1 |
| 64 | Autumn Bruno (UWW) def. Riley O'Donnell (LC) 6-1, 6-0 |
| 64 | Julia Baber (BU) def. Quinlyn Mack (UWSP) 0-6, 6-4, 10-2 |
| 64 | Yuki oda (GACW) def. Gracie Ha (UWW) 7-5, 6-4 |
| 64 | Morgan Nelson (UWEC) def. Cecile Fuchs (EC) 6-4, 6-4 |
| 64 | Georgia Nissen (WC) def. Bethany Marshall (UWW) 6-3, 6-1 |
| 64 | Alli Laux (GACW) def. Renata Martinez (LC) 6-0, 6-2 |
| 64 | Xingchen Liu (CAR) def. Madison Packer (GACW) 6-1, 6-0 |
| 64 | Ashley Samuta (CWI) def. Georgia Nissen (WC) 6-1, 6-0 |
| 32 | Autumn Bruno (UWW) def. Julia Baber (BU) 6-0, 6-2 |
| 32 | Yuki oda (GACW) def. Morgan Nelson (UWEC) 6-4, 6-2 |
| 32 | Alli Laux (GACW) def. Mira Manchanda (GC) 7-5, 1-0, retired |
| 32 | Xingchen Liu (CAR) def. Ashley Samuta (CWI) 6-1, 6-2 |
| 16 | Autumn Bruno (UWW) def. Yuki oda (GACW) 6-2, 6-3 |
| 16 | Alli Laux (GACW) def. Xingchen Liu (CAR) 6-4, 6-2 |
| 8 | Autumn Bruno (UWW) def. Alli Laux (GACW) 6-3, 6-3 |
| 128 | Sofia Sackett (LC) def. Elizabeth Trevathan (MC) 6-4, 5-7, 10-7 |
| 128 | Natalie Kaiser (WC) def. Mary Enright (GACW) 6-3, 7-5 |
| 128 | Sophia Jonas (CSB) def. Pavla Yakimova (GACW) 6-2, 3-6, 10-7 |
| 128 | Courtney Day (UWW) def. Jewel Lindwall (EC) 7-5, 6-4 |
| 128 | Renata Hernandez (GACW) def. Haley Quick (UWS) 6-0, 6-0 |
| 128 | Elsa Joly (SCU) def. Morgan Dekan (UWEC) 7-5, 6-0 |
| 128 | Maddie Dosser (SMU) def. Bella Tranquilino (SC) 6-4, 6-2 |
| 128 | Sophia DeLos Reyes (GC) def. Elise Bierbaum (BU) 4-6, 6-1, 10-6 |
| 128 | Faith Nosek (UWW) def. Tristan Jantz (UWSP) 7-6, 6-1 |
| 64 | Sofia Sackett (LC) def. Natalie Kaiser (WC) 6-0, 6-0 |
| 64 | Clare Palen (UWEC) def. Susi Hu (UWLC) 6-0, 6-0 |
| 64 | Maddie Schultz (COE) def. Pavla Yakimova (GACW) 7-5, 6-3 |
| 64 | Irene Augusti (GC) def. Courtney Day (UWW) 4-6, 6-3, 14-12 |
| 64 | Renata Hernandez (GACW) def. Elsa Joly (SCU) 6-3, 6-2 |
| 64 | Kasey Schlicht (UWW) def. Maddie Dosser (SMU) 6-0, 6-2 |
| 64 | Faith Nosek (UWW) def. Sophia DeLos Reyes (GC) 6-2, 6-4 |
| 64 | Allison Szalay (GACW) def. Cheyanne Phillips (CWI) 6-0, 6-0 |
| 32 | Clare Palen (UWEC) def. Sofia Sackett (LC) 6-2, 6-1 |
| 32 | Maddie Schultz (COE) def. Irene Augusti (GC) 6-1, 7-6 |
| 32 | Kasey Schlicht (UWW) def. Renata Hernandez (GACW) 6-0, 6-2 |
| 32 | Allison Szalay (GACW) def. Faith Nosek (UWW) 6-2, 6-1 |
| 16 | Clare Palen (UWEC) def. Maddie Schultz (COE) 6-4, 6-4 |
| 16 | Allison Szalay (GACW) def. Kasey Schlicht (UWW) 6-0, 6-4 |
| 8 | Allison Szalay (GACW) def. Clare Palen (UWEC) 3-6, 6-3, 6-0 |
| 128 | Katie Kearney (UWLC) def. Ellie Murphy (EC) 5-6, 6-0, 10-4 |
| 128 | Raquel Egge (CCM) def. Mallory Moen (CWI) 6-3, 6-3 |
| 128 | Julia Moreno (LC) def. Brooke Haddorff (GACW) 6-0, 6-4 |
| 128 | Hayley Trebil (GACW) def. Jennifer Larson (CENW) 6-0, 7-5 |
| 128 | Erin Jarvis (GC) def. Isabel Schwabe (LOR) 4-6, 6-2, 10-5 |
| 128 | Giovanna Mendes (CAR) def. Savannah Haugen (MC) 6-2, 6-2 |
| 128 | Molly Asfeld (UWW) def. Greta Nesbit (CSB) 6-1, 6-2 |
| 128 | Anna Lee (UWEC) def. Emma Thole (BU) 7-6, 6-2 |
| 64 | Raquel Egge (CCM) def. Katie Kearney (UWLC) 6-4, 6-2 |
| 64 | Olga Zubarieva (STO) def. Julia Moreno (LC) 7-6, 6-1 |
| 64 | Meadow LaDuke (WC) def. Hayley Trebil (GACW) 6-4, 6-1 |
| 64 | Gianna Nikoleit (UWW) def. Erin Jarvis (GC) 6-1, 7-5 |
| 64 | Molly Austin (GACW) def. Giovanna Mendes (CAR) 6-0, 6-0 |
| 64 | Ioanna Giannakou (GC) def. Molly Asfeld (UWW) 6-4, 6-4 |
| 64 | Martina Garcia (SSC) def. Lili Elofson (GACW) 6-7, 6-2, 10-4 |
| 64 | Kelsey Dorr (SCU) def. Anna Lee (UWEC) 6-0, 6-4 |
| 32 | Olga Zubarieva (STO) def. Raquel Egge (CCM) 6-0, 6-1 |
| 32 | Gianna Nikoleit (UWW) def. Meadow LaDuke (WC) 6-0, 6-1 |
| 32 | Molly Austin (GACW) def. Ioanna Giannakou (GC) 6-2, 7-5 |
| 32 | Kelsey Dorr (SCU) def. Martina Garcia (SSC) 6-0, 6-1 |
| 16 | Gianna Nikoleit (UWW) def. Olga Zubarieva (STO) 4-6, 6-2, 6-3 |
| 16 | Kelsey Dorr (SCU) def. Molly Austin (GACW) 6-2, 6-1 |
| 8 | Kelsey Dorr (SCU) def. Gianna Nikoleit (UWW) 6-1, 2-6, 6-2 |
| 4 | Kelsey Dorr (SCU) def. Allison Szalay (GACW) 6-2, 1-6, 6-2 |
| 2 | Sabring Tang (GC) def. Kelsey Dorr (SCU) 7-5, 6-7, 6-2 |
