| 128 | Ginger Valentine BYE |
| 128 | Courtney Day (UWW-W) def. Shira Busch (UWL) 7-6, 6-3 |
| 128 | Lily Fredricks (CSB) def. Emmaline Fievet (CCW) 6-0, 6-3 |
| 128 | Sydney Rott (BU) def. Emma Merrill (SNC) 6-0, 6-1 |
| 128 | Apostoli, Giana BYE |
| 128 | Jenna Forknell (CC) def. Picard, Alicia (EC) 7-5, 6-4 |
| 128 | Krueger, Grace BYE |
| 128 | Karleigh Schilling (CENW) def. Rebekah Spinegelhoff (CUW) 6-1, 6-1 |
| 128 | Abby Nielsen BYE |
| 128 | Ingrid Goldstrand (BU) def. Emma Lassen (COE) 6-1, 6-1 |
| 128 | Paige McLeod BYE |
| 128 | Allie Prokosch BYE |
| 128 | Wiedmeier,Alisha BYE |
| 128 | Megan Karrow (GACW) def. Claire Buehler (MAC) 1-6, 6-3, 6-1 |
| 128 | Olivia Kraft (UWW-W) def. Hannah Swajanen (SNC) 6-1, 6-1 |
| 128 | Lindsey Henderson (UWECW) def. Lauren O'Brian (WARWT) 6-1, 6-1 |
| 128 | Maggie Riermann BYE |
| 128 | Kaitlyn Kalinka BYE |
| 128 | Dorr,Bri BYE |
| 128 | Emma Gaydos (GCW) def. Cassandra Barr (SMU) 7-6, 6-3 |
| 128 | Sabrina Palavra BYE |
| 128 | Lauren Paulson (SNC) def. Emily King (SIMW) 7-5, 6-1 |
| 128 | Emily Norman (GACW) def. Tessa Rugg (HAM) 6-0, 6-0 |
| 128 | Emily Cooper (UWECW) def. Madison Overmann (WARWT) 6-3, 6-0 |
| 128 | Kimberly Steinert BYE |
| 128 | Verbauwhede, Sydney (EC) def. Ramsey, Sam (CARTHAGE) 6-3, 7-6 |
| 128 | Laurel Hickey BYE |
| 128 | Elsie Ha Ella White |
| 128 | Simona Potockova BYE |
| 128 | Shelby Cook (LUTW) def. Emily Otteson (STO) 7-6, 6-3 |
| 128 | Tessa Rahrick BYE |
| 128 | Ali Hickey (GCW) def. Ricki Budewitz (CUW) 6-1, 6-1 |
| 128 | Ptak, Jen (CARTHAGE) def. Nicole Pfotenhauer (SNC) 7-5, 7-5 |
| 128 | Sofia Walker (SCU) def. Lauren Witt (UWL) 6-4, 6-1 |
| 128 | Heineman, Kai BYE |
| 128 | Rachel Lindrud BYE |
| 128 | Sabrina Barboza BYE |
| 128 | Izzy Green (GCW) def. Mia Andrae (CUW) 6-0, 6-2 |
| 128 | Mackenzie Novak BYE |
| 128 | Verbauwhede, Ally BYE |
| 128 | Raquel Egge BYE |
| 128 | Maura Andersen (UWL) def. Knoll,Maddy (SCU) 6-0, 6-2 |
| 128 | Anna Stutz BYE |
| 128 | Amalin Sorajja (MAC) def. Kelsey Kruse (LUTW) 6-3, 1-6, 6-3 |
| 128 | Maddie Hartley (CENW) def. Morgan Moxon (SNC) 6-2, 6-0 |
| 128 | Elaine Franta (UWECW) def. Rylee Schurman (BU) 6-3, 7-5 |
| 128 | Sabrina Tang BYE |
| 128 | Jessica Minkov BYE |
| 128 | BYE BYE |
| 128 | Devon Bourget BYE |
| 128 | Taylor Hopkins BYE |
| 128 | Kelsey Dorr BYE |
| 128 | Natali Sorajja (MAC) def. Abbey Nelson (CCW) 6-1, 6-0 |
| 128 | Jennifer Mies (CUW) def. Hannah Riegle (UWL) 6-1, 6-0 |
| 128 | Sophia De Los Reyes (GCW) def. Carew, Alyssa (EC) 6-2, 6-1 |
| 128 | Natalie Wijesinghe BYE |
| 128 | Kellie Hierl (UWL) def. Sydney Douglas (GACW) 6-2, 6-2 |
| 128 | Kayla McIver BYE |
| 128 | Eliza Sundermann (COE) def. Colleen Lois (SMU) 7-5, 3-6, 6-4 |
| 128 | Paige McLeod BYE |
| 128 | Greta Prokosch (STO) def. Hannah Fox (WARWT) 6-2, 6-0 |
| 128 | LaBelle, Ashley (CARROLL) def. Anderson, Rachel (CARTHAGE) 6-4, 6-1 |
| 128 | Madeline Slicker BYE |
| 128 | Grace Riermann BYE |
| 64 | Grace Riermann (BU) def. Madeline Slicker (UWW-W) 6-0, 6-0 |
| 64 | LaBelle, Ashley (CARROLL) def. Greta Prokosch (STO) 6-4, 6-2 |
| 64 | Paige McLeod (UST) def. Colleen Lois (SMU) 6-2, 6-3 |
| 64 | Kellie Hierl (UWL) def. Kayla McIver (CSB) 7-5, 6-0 |
| 64 | Natalie Wijesinghe (UWECW) def. Sophia De Los Reyes (GCW) 7-6, 6-3 |
| 64 | Natali Sorajja (MAC) def. Jennifer Mies (CUW) 6-1, 6-0 |
| 64 | Kelsey Dorr (SCU) def. Taylor Hopkins (HAM) 6-0, 6-0 |
| 64 | Jessica Minkov (UWW-W) def. Devon Bourget (LUTW) 6-3, 6-2 |
| 64 | Sofia Walker (SCU) def. Ptak, Jen (CARTHAGE) 6-0, 6-1 |
| 64 | Paige Nierman (UWW-W) def. Heineman, Kai (EC) 6-2, 6-0 |
| 64 | Sabrina Barboza (STO) def. Rachel Lindrud (GACW) 6-4, 4-6, 7-6 |
| 64 | Mackenzie Novak (BU) def. Izzy Green (GCW) 6-1, 6-1 |
| 64 | Raquel Egge (CC) def. Verbauwhede, Ally (EC) 6-4, 6-4 |
| 64 | Anna Stutz (GACW) def. Maura Andersen (UWL) 6-1, 6-2 |
| 64 | Amalin Sorajja (MAC) def. Maddie Hartley (CENW) 7-5, 6-1 |
| 64 | Sabrina Tang (GCW) def. Elaine Franta (UWECW) 6-0, 6-1 |
| 64 | Maggie Riermann (BU) def. Kaitlyn Kalinka (UWL) 6-2, 6-4 |
| 64 | Dorr,Bri (SCU) def. Emma Gaydos (GCW) 6-2, 2-6, 6-2 |
| 64 | Sabrina Palavra (UWW-W) def. Lauren Paulson (SNC) 6-1, 6-0 |
| 64 | Emily Norman (GACW) def. Madison Overmann (WARWT) 6-2, 6-1 |
| 64 | Kimberly Steinert (UWL) def. Verbauwhede, Sydney (EC) 6-0, 6-2 |
| 64 | Elsie Ha (UWW-W) def. Laurel Hickey (MAC) 6-4, 6-1 |
| 64 | Shelby Cook (LUTW) def. Simona Potockova (GACW) 7-5, 6-2 |
| 64 | Ali Hickey (GCW) def. Tessa Rahrick (CSB) 6-0, 6-1 |
| 64 | Ginger Valentine (GACW) def. Courtney Day (UWW-W) 6-0, 6-0 |
| 64 | Lily Fredricks (CSB) def. Sydney Rott (BU) 4-6, 6-2, 6-1 |
| 64 | Apostoli, Giana (CARTHAGE) def. Jenna Forknell (CC) 7-6, 6-0 |
| 64 | Krueger, Grace (CARROLL) def. Karleigh Schilling (CENW) 3-6, 6-3, 6-3 |
| 64 | Abby Nielsen (GCW) def. Ingrid Goldstrand (BU) 6-3, 6-2 |
| 64 | Paige McLeod (UST) def. Allie Prokosch (STO) 6-3, 6-7, 6-3 |
| 64 | Megan Karrow (GACW) def. Wiedmeier,Alisha (SCU) 6-3, 6-4 |
| 64 | Olivia Kraft (UWW-W) def. Lindsey Henderson (UWECW) 6-2, 6-4 |
| 32 | Olivia Kraft (UWW-W) def. Megan Karrow (GACW) 6-1, 6-1 |
| 32 | Abby Nielsen (GCW) def. Paige McLeod (UST) 6-0, 6-0 |
| 32 | Apostoli, Giana (CARTHAGE) def. Krueger, Grace (CARROLL) 6-3, 6-1 |
| 32 | Ginger Valentine (GACW) def. Lily Fredricks (CSB) 6-2, 6-1 |
| 32 | Maggie Riermann (BU) def. Dorr,Bri (SCU) 6-3, 6-0 |
| 32 | Sabrina Palavra (UWW-W) def. Emily Norman (GACW) 6-0, 6-4 |
| 32 | Kimberly Steinert (UWL) def. Elsie Ha (UWW-W) 6-0, 6-1 |
| 32 | Ali Hickey (GCW) def. Shelby Cook (LUTW) 6-3, 6-7, 6-3 |
| 32 | Paige Nierman (UWW-W) def. Sofia Walker (SCU) 6-0, 6-1 |
| 32 | Mackenzie Novak (BU) def. Sabrina Barboza (STO) 7-6, 6-3 |
| 32 | Anna Stutz (GACW) def. Raquel Egge (CC) 6-1, 6-0 |
| 32 | Sabrina Tang (GCW) def. Amalin Sorajja (MAC) 6-1, 6-2 |
| 32 | Jessica Minkov (UWW-W) def. Kelsey Dorr (SCU) 6-3, 6-2 |
| 32 | Natalie Wijesinghe (UWECW) def. Natali Sorajja (MAC) 6-0, 6-0 |
| 32 | Kellie Hierl (UWL) def. Taylor Mcleod (UST) 6-4, 6-1 |
| 32 | Grae Riermann (BU) def. LaBelle, Ashley (CARROLL) 6-1, 6-0 |
| 16 | Grae Riermann (BU) def. Taylor Mcleod (UST) 6-2, 6-0 |
| 16 | Natalie Wijesinghe (UWECW) def. Jessica Minkov (UWW-W) 6-2, 6-1 |
| 16 | Paige Nierman (UWW-W) def. Mackenzie Novak (BU) 7-6, 3-6, 6-1 |
| 16 | Sabrina Tang (GCW) def. Anna Stutz (GACW) 6-0, 6-1 |
| 16 | Ginger Valentine (GACW) def. Apostoli, Giana (CARTHAGE) 6-3, 6-3 |
| 16 | Abby Nielsen (GCW) def. Olivia Kraft (UWW-W) 4-6, 6-3, 6-4 |
| 8 | Ginger Valentine (GACW) def. Abby Nielsen (GCW) 6-4, 6-2 |
| 8 | Kimberly Steinert (UWL) def. Sabrina Palavra (UWW-W) 6-4, 6-1 |
| 8 | Paige Nierman (UWW-W) def. Sabrina Tang (GCW) 6-4, 6-4 |
| 8 | Natalie Wijesinghe (UWECW) def. Grae Riermann (BU) 7-5, 6-1 |
| C32 | Emmaline Fievet (CCW) def. Emma Merrill (SNC) 6-3, 6-2 |
| C32 | Wiedmeier,Alisha (SCU) def. Claire Buehler (MAC) 6-2, 6-1 |
| C32 | Lauren O'Brian (WARWT) def. Hannah Swajanen (SNC) 6-2, 6-0 |
| C32 | Emily Cooper (UWECW) def. Tessa Rugg (HAM) 6-1, 6-0 |
| C32 | Simona Potockova (GACW) def. Emily Otteson (STO) 6-3, 6-1 |
| C32 | Ricki Budewitz (CUW) def. Tessa Rahrick (CSB) 6-4, 6-1 |
| C16 | Ginger Valentine (GACW) def. Ricki Budewitz (CUW) 6-7, 6-4, 11-9 |
| C16 | Ramsey, Sam (CARTHAGE) def. Rachel Lindrud (GACW) 6-3, 6-4 |
| C16 | Emily King (SIMW) def. Emily Cooper (UWECW) 2-6, 6-3, 10-8 |
| C16 | Cassandra Barr (SMU) def. Kaitlyn Kalinka (UWL) 6-3, 4-6, 10-6 |
| C16 | Wiedmeier,Alisha (SCU) def. Lauren O'Brian (WARWT) 6-2, 6-3 |
| C16 | Emma Lassen Allie Prokosch wd |
| C16 | Picard, Alicia (EC) def. Rebekah Spinegelhoff (CUW) 6-1, 6-2 |
| C16 | Shira Busch (UWL) def. Emmaline Fievet (CCW) 6-0, 6-1 |
| C8 | Shira Busch (UWL) def. Picard, Alicia (EC) 6-1, 6-3 |
| C8 | Allie Prokosch (STO) def. Wiedmeier,Alisha (SCU) 6-4, 6-4 |
| C8 | Emily King (SIMW) def. Cassandra Barr (SMU) 6-2, 6-1 |
| C8 | Simona Potockova (GACW) def. Ramsey, Sam (CARTHAGE) 6-7, 6-4, 11-9 |
| C4 | Simona Potockova Emily King |
| C4 | Allie Prokosch Shira Busch |
| C32 | Nicole Pfotenhauer (SNC) def. Lauren Witt (UWL) 0-6, 6-3, 10-2 |
| C32 | Kelsey Kruse (LUTW) def. Morgan Moxon (SNC) 6-3, 6-3 |
| C32 | Hannah Riegle (UWL) def. Abbey Nelson (CCW) 6-2, 6-4 |
| C32 | Kayla McIver (CSB) def. Sydney Douglas (GACW) 7-6, 2-6, 14-12 |
| C32 | Anderson, Rachel (CARTHAGE) def. Hannah Fox (WARWT) 6-3, 6-0 |
| C16 | Madeline Slicker (UWW-W) def. Anderson, Rachel (CARTHAGE) 6-1, 6-0 |
| C16 | Kayla McIver (CSB) def. Eliza Sundermann (COE) 6-2, 6-2 |
| C16 | Carew, Alyssa (EC) def. Hannah Riegle (UWL) 6-1, 6-4 |
| C16 | Devon Bourget (LUTW) def. Taylor Hopkins (HAM) by default |
| C16 | Kelsey Kruse (LUTW) def. Rylee Schurman (BU) 6-2, 5-7, 10-7 |
| C16 | Knoll,Maddy (SCU) def. Verbauwhede, Ally (EC) 6-3, 7-6 |
| C16 | Mia Andrae BYE |
| C16 | Heineman, Kai (EC) def. Nicole Pfotenhauer (SNC) 6-1, 6-0 |
| C8 | Heineman, Kai (EC) def. Mia Andrae (CUW) 6-4, 6-2 |
| C8 | Knoll,Maddy (SCU) def. Kelsey Kruse (LUTW) 7-5, 6-3 |
| C8 | Devon Bourget (LUTW) def. Carew, Alyssa (EC) 6-2, 6-3 |
| C8 | Madeline Slicker (UWW-W) def. Kayla McIver (CSB) 6-3, 6-4 |
| 4 | Ginger Valentine (GACW) def. Kimberly Steinert (UWL) 6-3, 6-2 |
| 4 | Grae Riermann (BU) def. Paige Nierman (UWW-W) 6-3, 6-1 |
| 2 | Grae Riermann (BU) def. Ginger Valentine (GACW) 7-5, 6-4 |
