| 128 | Emily Cooper (UWEC) def. Stephanie Bush (COEW) 6-3, 6-3 |
| 128 | Hannah Borgh (GACW) def. Allie Miller (UWW-W) 3-6, 7-5, 7-5 |
| 128 | Britta Pressler (LUTW) def. Emma Gaydos (GCW) 6-7, 6-2, 6-0 |
| 128 | Budewitz, Ricki (CUW) def. Samantha Koppa (UWO) 6-4, 6-2 |
| 128 | Verbauwhede, Sydney (EC) def. Allie Miller (UWW-W) 6-4, 6-3 |
| 128 | Elizabeth Fink (CSS) def. Karleigh Schilling (CENW) 3-6, 6-2, 6-2 |
| 128 | Abbey Nelson (CCW) def. Ellie Bergstrand (STO) 4-6, 7-6, 6-4 |
| 128 | Mogden, Shanna (CARROLL) def. Emily King (SIMW) 7-5, 6-4 |
| 128 | Natali Sorajja (MAC) def. Pham, Nia (CARTHAGE) 3-6, 6-1, 6-1 |
| 128 | Kaitlyn Kalinka (UWL) def. Olivia Kraft (UWW-W) 1-6, 6-4, 7-5 |
| 128 | Sydney Rott (BUW) def. Cami Gricius (CCW) 6-7, 6-2, 6-2 |
| 128 | Jill Weinzettel (BUW) def. Hannah Peters (UWO) 6-1, 6-1 |
| 128 | Kellis Brandt (STO) def. Colleen Lois (SMU) 6-1, 7-5 |
| 128 | Sabrina Palavra (UWW-W) def. Isabel Green (GCW) 7-5, 6-4 |
| 128 | Bri Dorr (SCU) def. Natalie Pieterick (HU) 6-0, 6-2 |
| 128 | Shelby Cook (LUTW) def. Lily Hamilton (GCW) 6-1, 6-3 |
| 128 | Alyssa Boyer (UWL) def. Taylor Kopfer (BUW) 6-1, 6-2 |
| 128 | Cassandra Barr (SMU) def. Eliza Sundermann (COEW) 2-6, 6-2, 6-1 |
| 128 | Meg Witt (SNC) def. Emma Baker (MAC) 6-4, 6-7, 6-3 |
| 128 | Nikki Pasketwitz (UNWW) def. Raquel Ruiz (CCW) 6-2, 6-2 |
| 128 | Madeline Slicker (UWW-W) def. Carew, Alyssa (EC) 6-4, 4-6, 7-5 |
| 128 | Jenna Forknell (CC) def. Kayla Reusche (SIMW) 6-3, 6-2 |
| 128 | Sabrina Barboza (STO) def. Maura Andersen (UWL) 6-3, 1-6, 6-1 |
| 128 | Sheila Sullivan (GACW) def. Michelle Spicer (UWO) 6-0, 6-1 |
| 128 | Elise Ha (UWW-W) def. Tessa Rugg (HU) 6-0, 6-1 |
| 128 | Devon Bourget (LUTW) def. Alisha Wiedmeir (SCU) 7-6, 6-4 |
| 128 | Favia, Natalie (CARTHAGE) def. Jenna Stewart (CENW) 7-6, 6-2 |
| 128 | Sarah Benoy (UST-WOM) def. Kelly, Bailey (CUW) 6-1, 6-2 |
| 128 | Laurel Hickey (MAC) def. Julia Boeve (SMU) 1-6, 6-4, 6-2 |
| 128 | Jessica Minkov (UWW-W) def. Isabella Hoppenjans (COEW) 6-0, 6-3 |
| 128 | Elizabeth Yim (CAR) def. Elise Hauer (CSS) 6-4, 6-3 |
| 64 | Gabi Kitchell (UWW-W) def. Emily Cooper (UWEC) 6-1, 6-1 |
| 64 | Laura Sonday (SMU) def. Hannah Borgh (GACW) 3-6, 6-0, 7-5 |
| 64 | Emmanouil, Angelica (CARTHAGE) def. Britta Pressler (LUTW) 6-1, 6-4 |
| 64 | Maggie Riermann (BUW) def. Verbauwhede, Sydney (EC) 6-3, 6-0 |
| 64 | Yelena Hallman (CAR) def. Elizabeth Fink (CSS) 6-3, 6-2 |
| 64 | Lilly Hartman (GACW) def. Abbey Nelson (CCW) 6-2, 6-3 |
| 64 | Sadie Spahn (UWL) def. Mogden, Shanna (CARROLL) 6-0, 6-1 |
| 64 | Ginger Valentine (GACW) def. Natali Sorajja (MAC) 6-2, 6-1 |
| 64 | Solberg, Ashley (CUW) def. Kaitlyn Kalinka (UWL) 6-0, 7-5 |
| 64 | Lauren French (COEW) def. Sydney Rott (BUW) 6-2, 6-3 |
| 64 | Jill Weinzettel (BUW) def. Anna Leary (UST-WOM) 7-6, 6-1 |
| 64 | Sabrina Palavra (UWW-W) def. Hayley Clope (UWL) 6-4, 6-4 |
| 64 | Natalie Wijesinghe (UWEC) def. Bri Dorr (SCU) 6-0, 6-2 |
| 64 | Anna Stutz (GACW) def. Hannah Fox (WARTBURG) 6-0, 6-1 |
| 64 | Shelby Cook (LUTW) def. Ally Thorsen (GACW) 6-1, 6-3 |
| 64 | Julie Martin (UWW-W) def. Alyssa Boyer (UWL) 6-4, 6-0 |
| 64 | Cassandra Barr (SMU) def. Alyssa LEffler (UWO) 6-2, 6-3 |
| 64 | Ali Kopiecki (HU) def. Meg Witt (SNC) 6-1, 6-0 |
| 64 | Ramsey, Sam (CARTHAGE) def. Nikki Pasketwitz (UNWW) 0-6, 7-5, 6-4 |
| 64 | Simona Potockova (GACW) def. Madeline Slicker (UWW-W) 6-2, 6-4 |
| 64 | Mackenzie Novak (BUW) def. Jenna Forknell (CC) 6-1, 6-1 |
| 64 | Sonya Romanenko (CAR) def. Sabrina Barboza (STO) 6-2, 6-3 |
| 64 | Molly Stewart (UWEC) def. Sheila Sullivan (GACW) 7-5, 6-1 |
| 64 | Grace Riermann (BUW) def. Elise Ha (UWW-W) 6-2, 6-1 |
| 64 | Devon Bourget (LUTW) def. Ashley Rottinghaus (WARTBURG) 6-4, 6-1 |
| 64 | Abby Nielsen (GCW) def. Favia, Natalie (CARTHAGE) 7-6, 6-2 |
| 64 | Sarah Benoy (UST-WOM) def. Picard, Alicia (EC) 6-2, 6-0 |
| 64 | Kimberly Steinert (UWL) def. Laurel Hickey (MAC) 6-0, 7-6 |
| 64 | Krueger, Grace (CARROLL) def. Jessica Minkov (UWW-W) 6-1, 6-3 |
| 64 | Bri Hartmann (GACW) def. Elizabeth Yim (CAR) 6-0, 6-1 |
| 32 | Gabi Kitchell (UWW-W) def. Laura Sonday (SMU) 6-0, 6-2 |
| 32 | Brooke Hapuku (UST-WOM) def. Emmanouil, Angelica (CARTHAGE) 6-2, 6-0 |
| 32 | Maggie Riermann (BUW) def. Yelena Hallman (CAR) 6-1, 6-2 |
| 32 | Lilly Hartman (GACW) def. Sadie Spahn (UWL) 6-3, 6-0 |
| 32 | Ginger Valentine (GACW) def. Solberg, Ashley (CUW) 6-0, 6-0 |
| 32 | Jill Weinzettel (BUW) def. Lauren French (COEW) 3-6, 6-4, 6-2 |
| 32 | Sabrina Palavra (UWW-W) def. Kellis Brandt (STO) 6-2, 7-5 |
| 32 | Natalie Wijesinghe (UWEC) def. Anna Stutz (GACW) 6-3, 6-2 |
| 32 | Julie Martin (UWW-W) def. Ally Thorsen (GACW) 6-1, 6-0 |
| 32 | Ali Kopiecki (HU) def. Cassandra Barr (SMU) 6-1, 6-0 |
| 32 | Simona Potockova (GACW) def. Ramsey, Sam (CARTHAGE) 2-6, 6-3, 6-2 |
| 32 | Sonya Romanenko (CAR) def. Mackenzie Novak (BUW) 6-2, 7-6 |
| 32 | Grace Riermann (BUW) def. Molly Stewart (UWEC) 6-1, 6-1 |
| 32 | Abby Nielsen (GCW) def. Devon Bourget (LUTW) 6-1, 6-2 |
| 32 | Kimberly Steinert (UWL) def. Sarah Benoy (UST-WOM) 7-5, 6-2 |
| 32 | Bri Hartmann (GACW) def. Krueger, Grace (CARROLL) 6-2, 6-2 |
| 16 | Gabi Kitchell (UWW-W) def. Brooke Hapuku (UST-WOM) 6-0, 6-0 |
| 16 | Maggie Riermann (BUW) def. Lilly Hartman (GACW) 6-0, 6-4 |
| 16 | Ginger Valentine (GACW) def. Jill Weinzettel (BUW) 6-0, 6-3 |
| 16 | Natalie Wijesinghe (UWEC) def. Sabrina Palavra (UWW-W) 6-3, 6-2 |
| 16 | Ali Kopiecki (HU) def. Julie Martin (UWW-W) 6-3, 6-7, 6-3 |
| 16 | Sonya Romanenko (CAR) def. Simona Potockova (GACW) 6-2, 6-1 |
| 16 | Grace Riermann (BUW) def. Abby Nielsen (GCW) 6-3, 6-1 |
| 16 | Bri Hartmann (GACW) def. Kimberly Steinert (UWL) 6-2, 6-2 |
| 8 | Gabi Kitchell (UWW-W) def. Maggie Riermann (BUW) 6-3, 6-3 |
| 8 | Ginger Valentine (GACW) def. Natalie Wijesinghe (UWEC) 6-2, 7-5 |
| 8 | Ali Kopiecki (HU) def. Sonya Romanenko (CAR) 6-4, 6-1 |
| 8 | Grace Riermann (BUW) def. Bri Hartmann (GACW) 6-3, 6-4 |
| 4 | Ginger Valentine (GACW) def. Gabi Kitchell (UWW-W) 6-4, 2-6, 6-3 |
| 4 | Ali Kopiecki (HU) def. Grace Riermann (BUW) 7-6, 7-5 |
| 2 | Ginger Valentine (GACW) def. Ali Kopiecki (HU) 6-0, 6-4 |
| C64 | Anna Leary (UST-WOM) def. Hannah Peters (UWO) 6-1, 6-0 |
| C64 | Hayley Clope (UWL) def. Isabel Green (GCW) 3-6, 6-0, 10-8 |
| C64 | Alyssa LEffler (UWO) def. Eliza Sundermann (COEW) 6-1, 6-1 |
| C64 | Picard, Alicia (EC) def. Kelly, Bailey (CUW) 7-6, 2-6, 11-9 |
| C32 | Stephanie Bush (COEW) def. Della Brown (MAC) by default |
| C32 | Emma Gaydos (GCW) def. Samantha Koppa (UWO) 3-6, 6-3, 10-5 |
| C32 | Allie Miller (UWW-W) def. Karleigh Schilling (CENW) 6-4, 2-6, 10-7 |
| C32 | Emily King (SIMW) def. Ellie Bergstrand (STO) 6-2, 7-6 (1-0) |
| C32 | Olivia Kraft (UWW-W) def. Pham, Nia (CARTHAGE) 3-6, 6-4, 10-7 |
| C32 | Anna Leary (UST-WOM) def. Cami Gricius (CCW) 6-3, 6-2 |
| C32 | Hayley Clope (UWL) def. Colleen Lois (SMU) 7-5, 6-2 |
| C32 | Hannah Fox (WARTBURG) def. Natalie Pieterick (HU) 6-0, 6-2 |
| C32 | Taylor Kopfer (BUW) def. Lily Hamilton (GCW) 6-3, 6-2 |
| C32 | Alyssa LEffler (UWO) def. Emma Baker (MAC) 6-1, 6-1 |
| C32 | Carew, Alyssa (EC) def. Raquel Ruiz (CCW) 7-6 (4-0), 6-2 |
| C32 | Maura Andersen (UWL) def. Kayla Reusche (SIMW) 6-2, 6-3 |
| C32 | Michelle Spicer (UWO) def. Tessa Rugg (HU) 6-3, 6-3 |
| C32 | Alisha Wiedmeir (SCU) def. Jenna Stewart (CENW) 6-2, 7-6 (1-0) |
| C32 | Julia Boeve (SMU) def. Picard, Alicia (EC) 6-0, 6-1 |
| C32 | Isabella Hoppenjans (COEW) def. Elise Hauer (CSS) by default |
| C16 | Emma Gaydos (GCW) def. Stephanie Bush (COEW) 6-0, 3-6, 10-5 |
| C16 | Allie Miller (UWW-W) def. Emily King (SIMW) 7-6 (3-0), 6-3 |
| C16 | Anna Leary (UST-WOM) def. Olivia Kraft (UWW-W) 7-6 (5-0), 6-2 |
| C16 | Hayley Clope (UWL) def. Hannah Fox (WARTBURG) 6-0, 6-0 |
| C16 | Alyssa LEffler (UWO) def. Taylor Kopfer (BUW) 6-4, 6-3 |
| C16 | Maura Andersen (UWL) def. Carew, Alyssa (EC) 6-4, 6-0 |
| C16 | Alisha Wiedmeir (SCU) def. Michelle Spicer (UWO) 6-0, 7-5 |
| C16 | Isabella Hoppenjans (COEW) def. Julia Boeve (SMU) 6-2, 4-6, 10-7 |
